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Assay by UT-Tokyo iGEM 2014

Figure 2 GFP fluorescence reporting the activities of various combinations of sigma factors and promoters. GFP was activated by 488 nm excitation laser and the activity was measured by 514 nm emission laser. Error bar shows average ± SE. N = 3.

As seen in Figure 2, ecf20 and ecf11 activated only their corresponding promoters. Leak from the promoters was also not observed. The result of the sample whose sigma factor was not induced by arabinose (ara-), which had little difference from the one induced by arabinose (ara+), may be caused by the leak of pBAD (BBa_I13453). The amount of the sigma factor leaked from pBAD may be over the switch-point of sigmoid curve indicating relation between sigma factor and its corresponding promoter. Thus, even if the amount of sigma factor changes by arabinose induction, corresponding activity of promoter may not change.

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